![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K4458010:Design
Designed by: Beth Tuck Group: iGEM22_East_Coast_BioCrew (2022-09-10)
CYP1A1-CYC1-Akaluc
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 119
Illegal NheI site found at 123
Illegal NheI site found at 127 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Human CYP1A1 promoter sequence had a region of high GC content that Twist was unable to synthesize in its original form. Therefore we used Twist's codon optimization service to modify the sequences EXCLUDING any highly conserved or annotated DRE elements.
Source
Human CYP1A1 Promoter, Genbank: NG_061374.1 Yeast CYC1 Yeast Promoter, GenBank: CP026288.1 Aakluc CDS, Addgene Plasmid #182212