Reporter

Part:BBa_K4458010:Design

Designed by: Beth Tuck   Group: iGEM22_East_Coast_BioCrew   (2022-09-10)


CYP1A1-CYC1-Akaluc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 119
    Illegal NheI site found at 123
    Illegal NheI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Human CYP1A1 promoter sequence had a region of high GC content that Twist was unable to synthesize in its original form. Therefore we used Twist's codon optimization service to modify the sequences EXCLUDING any highly conserved or annotated DRE elements.


Source

Human CYP1A1 Promoter, Genbank: NG_061374.1 Yeast CYC1 Yeast Promoter, GenBank: CP026288.1 Aakluc CDS, Addgene Plasmid #182212

References